Determination of ganciclovir in human plasma by ultra performance liquid chromatography-UV detection.
Clin Biochem
; 45(4-5): 309-14, 2012 Mar.
Article
en En
| MEDLINE
| ID: mdl-22245106
ABSTRACT
OBJECTIVES:
Implement a sensitive UHPLC method for the assay of ganciclovir in human plasma. DESIGN ANDMETHODS:
We developed and validated a chromatographic method coupled to ultraviolet detection for quantification of ganciclovir, with a short run time using a small volume of human plasma. Comparison of system performance was made with respect to analysis time, efficiency and sensitivity.RESULTS:
Correlation coefficients (r) of the calibration curves ranged from 0.999744 to 0.999784. Within-day and between-day imprecision and inaccuracy, specificity and recovery were also evaluated for validation. The method was precise and accurate and the retention time was 0.7 min. The calibration curves were linear between 0.5 and 30 µg/mL. There was a good correlation between HPLC and UHPLC techniques.CONCLUSIONS:
We developed a method that is currently applied in a clinical study assessing GCV plasma concentration variability after ganciclovir and valganciclovir administration.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Antivirales
/
Ganciclovir
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Clin Biochem
Año:
2012
Tipo del documento:
Article
País de afiliación:
España