Molecular cloning and characterization of plasma membrane- and vacuolar-type Naâº/H⺠antiporters of an alkaline-salt-tolerant monocot, Puccinellia tenuiflora.
J Plant Res
; 125(4): 587-94, 2012 Jul.
Article
en En
| MEDLINE
| ID: mdl-22270695
ABSTRACT
A better understanding of salt tolerance in plants might lead to the genetic engineering of crops that can grow in saline soils. Here we cloned and characterized plasma membrane and vacuolar Naâº/H⺠antiporters of a monocotyledonous alkaline-tolerant halophyte, Puccinellia tenuiflora. The predicted amino acid sequence of the transporters were very similar to those of orthologs in monocotyledonous crops. Expression analysis showed that (1) NHA was more strongly induced by NaCl in the roots of P. tenuiflora while in rice it was rather induced in the shoots, suggesting that the role of NHA in salt excretion from the roots partly accounts for the difference in the tolerance of the two species, and that (2) NHXs were specifically induced by NaHCO3 but not by NaCl in the roots of both species, suggesting that vacuolar-type Naâº/H⺠antiporters play roles in pH regulation under alkaline salt conditions. Overexpression of the antiporters resulted in increased tolerance of shoots to NaCl and roots to NaHCO3. Overexpression lines exhibited a lower Na⺠content and a higher K⺠content in shoots under NaCl treatments, leading to a higher Naâº/H⺠ratio.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Vacuolas
/
Membrana Celular
/
Intercambiadores de Sodio-Hidrógeno
/
Plantas Tolerantes a la Sal
/
Tolerancia a la Sal
Idioma:
En
Revista:
J Plant Res
Asunto de la revista:
BOTANICA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Japón