Tissue expression profiling using real-time PCR.
Curr Protoc Pharmacol
; Chapter 6: Unit 6.9, 2002 Nov.
Article
en En
| MEDLINE
| ID: mdl-22294081
ABSTRACT
The recent development of real-time PCR allows for the rapid and accurate quantitation of gene expression in cells and tissues. Real-time PCR instrumentation is designed for the simultaneous quantitation of gene expression from a few samples up to 384 samples. The normal tissue expression profile of a gene can provide useful insights into its potential role in normal physiological processes. When combined with the tissue expression profile of the gene in diseased tissues, information concerning the potential role in pathological processes can be determined. This unit describes a protocol to determine the relative abundance of mRNA in a panel of human tissues using real-time PCR.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ARN
/
ADN Complementario
/
Perfilación de la Expresión Génica
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Límite:
Humans
Idioma:
En
Revista:
Curr Protoc Pharmacol
Año:
2002
Tipo del documento:
Article
País de afiliación:
Estados Unidos