Ovarian cancer spheroids use myosin-generated force to clear the mesothelium.
Cancer Discov
; 1(2): 144-57, 2011 Jul.
Article
en En
| MEDLINE
| ID: mdl-22303516
ABSTRACT
Dissemination of ovarian tumors involves the implantation of cancer spheroids into the mesothelial monolayer on the walls of peritoneal and pleural cavity organs. Biopsies of tumors attached to peritoneal organs show that mesothelial cells are not present under tumor masses. We have developed a live, image-based in vitro model in which interactions between tumor spheroids and mesothelial cells can be monitored in real time to provide spatial and temporal understanding of mesothelial clearance. Here we provide evidence that ovarian cancer spheroids utilize integrin- and talin- dependent activation of myosin and traction force to promote mesothelial cells displacement from underneath a tumor cell spheroid. These results suggest that ovarian tumor cell clusters gain access to the sub-mesothelial environment by exerting force on the mesothelial cells lining target organs, driving migration and clearance of the mesothelial cells.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Ováricas
/
Esferoides Celulares
/
Epitelio
Límite:
Female
/
Humans
Idioma:
En
Revista:
Cancer Discov
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos