[Expression of hsa-miR-20a in human glioma tissues and its effect on the proliferation of human glioma cells in vitro].
Nan Fang Yi Ke Da Xue Xue Bao
; 32(2): 198-201, 2012 Feb.
Article
en Zh
| MEDLINE
| ID: mdl-22381757
ABSTRACT
OBJECTIVE:
To investigate miR-20a expression in human glioma and normal brain tissues and its effect on the proliferation of glioma cells in vitro.METHODS:
The expression of miR-20a was detected in human normal brain tissues and glioma tissues by real-time RT-PCR. miR-20a mimics were synthesized and transfected into U251 cells via liposome, and the cell proliferation were detected using MTT assay and flow cytometry.RESULTS:
The glioma tissues showed significantly up-regulated expression of miR-20a compared with normal brain tissues (P=0.035). The expression level of miR-20a was higher in high-grade than in low-grade gliomas. miR-20a mimics significantly enhanced the proliferation of U251 cells and the percentage of S-phase cells.CONCLUSION:
miR-20a shows potent effect in promoting the growth of glioma cells, suggesting its important role in the pathogenesis of human glioma.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Encefálicas
/
MicroARNs
/
Proliferación Celular
/
Glioma
Límite:
Adult
/
Female
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Humans
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Male
/
Middle aged
Idioma:
Zh
Revista:
Nan Fang Yi Ke Da Xue Xue Bao
Año:
2012
Tipo del documento:
Article
País de afiliación:
China