Proteomic analysis of Terminalia chebula extract-dependent changes in human lymphoblastic T cell protein expression.
J Med Food
; 15(7): 651-7, 2012 Jul.
Article
en En
| MEDLINE
| ID: mdl-22471968
ABSTRACT
Terminalia chebula is a native plant from southern Asia to southwestern China that is used in traditional medicine for the treatment of malignant tumors and diabetes. This plant also has antibacterial and immunomodulatory properties. The present study assessed T. chebula extract-dependent protein expression changes in Jurkat cells. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry and Ingenuity Pathways Analysis (IPA) were performed to assess protein expression and networks, respectively. A comparative proteomic profile was determined in T. chebula extract (50 µg/mL)-treated and control cells; the expressions of ß-tubulin, ring finger and CHY zinc finger domain containing 1, and insulin-like growth factor 1 receptor kinase were significantly down-regulated in T. chebula extract-treated Jurkat cells. Moreover, the molecular basis for the T. chebula extract-dependent protein expression changes in Jurkat cells was determined by IPA. Treatment with the T. chebula extract significantly inhibited nuclear factor-κB activity and affected the proteomic profile of Jurkat cells. The molecular network signatures and functional proteomics obtained in this study may facilitate the evaluation of potential antitumor therapeutic targets and elucidate the molecular mechanism of T. chebula extract-dependent effects in Jurkat cells.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Extractos Vegetales
/
Linfocitos T
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Leucemia de Células T
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Proteoma
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Terminalia
/
Fitoterapia
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Antineoplásicos Fitogénicos
Límite:
Humans
Idioma:
En
Revista:
J Med Food
Asunto de la revista:
CIENCIAS DA NUTRICAO
/
MEDICINA
Año:
2012
Tipo del documento:
Article