Mediation of Drosophila autosomal dosage effects and compensation by network interactions.
Genome Biol
; 13(4): r28, 2012 Apr 24.
Article
en En
| MEDLINE
| ID: mdl-22531030
ABSTRACT
BACKGROUND:
Gene dosage change is a mild perturbation that is a valuable tool for pathway reconstruction in Drosophila. While it is often assumed that reducing gene dose by half leads to two-fold less expression, there is partial autosomal dosage compensation in Drosophila, which may be mediated by feedback or buffering in expression networks.RESULTS:
We profiled expression in engineered flies where gene dose was reduced from two to one. While expression of most one-dose genes was reduced, the gene-specific dose responses were heterogeneous. Expression of two-dose genes that are first-degree neighbors of one-dose genes in novel network models also changed, and the directionality of change depended on the response of one-dose genes.CONCLUSIONS:
Our data indicate that expression perturbation propagates in network space. Autosomal compensation, or the lack thereof, is a gene-specific response, largely mediated by interactions with the rest of the transcriptome.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compensación de Dosificación (Genética)
/
Genes de Insecto
/
Drosophila
/
Redes Reguladoras de Genes
Límite:
Animals
Idioma:
En
Revista:
Genome Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos