Proteomics-based identification of α-enolase as a potential prognostic marker in cholangiocarcinoma.
Clin Biochem
; 45(10-11): 827-34, 2012 Jul.
Article
en En
| MEDLINE
| ID: mdl-22552009
ABSTRACT
OBJECTIVES:
To investigate the association of expression status of α-enolase (ENO1) and clinicopathological outcomes of CCA patients. DESIGN ANDMETHODS:
Two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to compare differential expressed protein profiles of four human CCA cell lines and H69, a non-malignant biliary cell line, as a control. Immunohistochemical analysis was carried out in tissue-microarray of human CCA tissues (n=301).RESULTS:
We identified ENO1 in all CCA cell lines but not H69 by proteomics based. About 75% of patients with CCA showed over-expression of ENO1 in hyperplastic bile duct and the tumors compared with that in tumor-adjacent normal tissue counterparts. Moreover, over-expression of ENO1 is significantly associated with poor prognosis and tumor invasion of CCA patients.CONCLUSIONS:
ENO1 may serve as a prognostic marker to monitor the disease progression of these patients.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfopiruvato Hidratasa
/
Neoplasias de los Conductos Biliares
/
Conductos Biliares Intrahepáticos
/
Biomarcadores de Tumor
/
Colangiocarcinoma
/
Proteómica
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Clin Biochem
Año:
2012
Tipo del documento:
Article
País de afiliación:
Tailandia