Plasmid-based Survivin shRNA and GRIM-19 carried by attenuated Salmonella suppresses tumor cell growth.
Asian J Androl
; 14(4): 536-45, 2012 Jul.
Article
en En
| MEDLINE
| ID: mdl-22580637
Persistent activation of Survivin and its overexpression contribute to the formation, progression and metastasis of several different tumor types. Therefore, Survivin is an ideal target for RNA interference mediated-growth inhibition. Blockade of Survivin using specific short hairpin RNAs (shRNA) can significantly reduce prostate tumor growth. RNA interference does not fully ablate target gene expression, owing to the idiosyncrasies associated with shRNAs and their targets. To enhance the therapeutic efficacy of Survivin-specific shRNA, we employed a combinatorial expression of Survivin-specific shRNA and gene associated with retinoid-interferon-induced mortality-19 (GRIM-19). Then, the GRIM-19 coding sequences and Survivin-specific shRNAs were used to create a dual expression plasmid vector and were carried by an attenuated strain of Salmonella enteric serovar typhimurium (S. typhimurium) to treat prostate cancer in vitro and in vivo. We found that the co-expressed Survivin-specific shRNA and GRIM-19 synergistically and more effectively inhibited prostate tumor proliferation and survival, when compared with treatment with either single agent alone in vitro and in vivo. This study has provided a novel cancer gene therapeutic approach for prostate cancer.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
/
6_ODS3_enfermedades_notrasmisibles
Problema de salud:
3_neglected_diseases
/
3_zoonosis
/
6_prostate_cancer
Asunto principal:
Neoplasias de la Próstata
/
Carcinoma
/
Terapia Genética
/
ARN Interferente Pequeño
/
Proteínas Reguladoras de la Apoptosis
/
Proteínas Inhibidoras de la Apoptosis
/
NADH NADPH Oxidorreductasas
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Asian J Androl
Asunto de la revista:
MEDICINA REPRODUTIVA
/
UROLOGIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
China