Comparison of a gel microcolumn assay with the conventional tube test for red blood cell alloantibody titration.
Transfusion
; 53(4): 811-5, 2013 Apr.
Article
en En
| MEDLINE
| ID: mdl-22804101
ABSTRACT
BACKGROUND:
Titration is a semiquantitative method used to estimate red blood cell (RBC) alloantibody reactivity. The conventional tube test (CTT) technique is the traditional method for performing titration studies. The gel microcolumn assay (GMA) is also a sensitive method to detect RBC alloantibodies. The aim of this study was to compare a GMA with the CTT technique in the performance of Rh and K alloantibody titration. STUDY DESIGN ANDMETHODS:
Patient serum samples that contained an RBC alloantibody with a singular specificity were identified by routine blood bank workflow. Parallel titration studies were performed on these samples by both the CTT method and a GMA (ID-Micro Typing System anti-IgG gel card, Micro Typing Systems, Inc., an Ortho-Clinical Diagnostics Company).RESULTS:
Forty-eight samples were included, including 11 anti-D, five anti-c, 13 anti-E, one anti-C, three anti-e, and 15 anti-K. Overall, the two methods generated identical results in 21 of 48 samples. For 42 samples (87.5%) the two methods generated results that were within one serial dilution, and for the remaining six samples, results were within two dilutions.CONCLUSION:
GMA systems may perform comparably to the CTT in titrating alloantibodies to Rh and Kell antigens.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sistema del Grupo Sanguíneo Rh-Hr
/
Tipificación y Pruebas Cruzadas Sanguíneas
/
Eritrocitos
/
Isoanticuerpos
/
Sistema del Grupo Sanguíneo de Kell
Tipo de estudio:
Evaluation_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Transfusion
Año:
2013
Tipo del documento:
Article
País de afiliación:
Estados Unidos