An environmental epigenetic study of ADRB2 5'-UTR methylation and childhood asthma severity.
Clin Exp Allergy
; 42(11): 1575-81, 2012 Nov.
Article
en En
| MEDLINE
| ID: mdl-22862293
ABSTRACT
BACKGROUND:
Beta-2 adrenergic receptor (ADRB2) is the primary target of both short- and long-acting beta-agonist asthma medications. ADRB2 5'-UTR methylation changes in blood have the potential to act as a surrogate biomarker of responsiveness to beta-agonist treatment and childhood asthma severity.OBJECTIVE:
To study the association between ADRB2 5'-UTR methylation, NO (2) exposure and childhood asthma severity.METHODS:
We compared ADRB2 5'-UTR methylation levels in blood between 60 children with mild asthma and 122 children with severe asthma using methylation-specific PCR. We also investigated potential joint effects between NO (2) exposure and ADRB2 5'-UTR methylation.RESULTS:
We found a significant association between intermediate (OR 4.11, 95% CI 1.58-10.73) and high levels (OR 7.63, 95% CI 3.02-19.26) of ADRB2 methylation and severe childhood asthma. In addition, we found a significant association between indoor exposure to NO (2) , an air pollutant and known asthmogen, and severe asthma among children exhibiting high ADRB2 methylation (OR 4.59, 95% CI 1.03-20.55) but no association among children exhibiting low levels of ADRB2 methylation (OR 0.35, 95% CI 0.01-14.13). CONCLUSIONS AND CLINICAL RELEVANCE These findings support the potential use of ADRB2 5'-UTR methylation as a biomarker of both asthma severity and risk for NO (2) -associated asthma exacerbations in children, and present the first evidence of an epigenetic link between an important environmental exposure and childhood asthma severity.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Asma
/
Receptores Adrenérgicos beta 2
/
Metilación de ADN
/
Regiones no Traducidas 5'
/
Epigénesis Genética
/
Exposición a Riesgos Ambientales
/
Dióxido de Nitrógeno
Tipo de estudio:
Etiology_studies
/
Risk_factors_studies
Límite:
Adolescent
/
Child
/
Child, preschool
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Clin Exp Allergy
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos