Dopaminergic differentiation of human pluripotent cells.
Curr Protoc Stem Cell Biol
; Chapter 1: Unit1H.6, 2012 Aug.
Article
en En
| MEDLINE
| ID: mdl-22872424
ABSTRACT
Here we describe protocols for the dopaminergic differentiation of pluripotent stem cells. We have optimized and compared two distinct protocols, both of which are chemically defined and applicable to both embryonic and induced pluripotent stem cells. First, we present a five-step method based on rosette formation (Basic Protocol 1); then we describe a monolayer paradigm based on inhibition of alternate developmental pathways (Basic Protocol 2). Directed differentiation of pluripotent cells into specific cell types is a crucial step towards understanding human development and realizing the biomedical relevance of these cells, whether for replacement therapy or disease modeling.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Diferenciación Celular
/
Técnicas de Cultivo de Célula
/
Células Madre Pluripotentes
/
Neuronas Dopaminérgicas
Tipo de estudio:
Guideline
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Curr Protoc Stem Cell Biol
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos