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Evaluation of DNA extraction methods of rumen microbial populations.
Villegas-Rivera, Gabriela; Vargas-Cabrera, Yevani; González-Silva, Napoleón; Aguilera-García, Florentino; Gutiérrez-Vázquez, Ernestina; Bravo-Patiño, Alejandro; Cajero-Juárez, Marcos; Baizabal-Aguirre, Víctor Manuel; Valdez-Alarcón, Juan José.
Afiliación
  • Villegas-Rivera G; Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Km 9.5 carretera Morelia-Zinapécuaro, La Palma, C.P. 58893 Tarímbaro, Michoacán, Mexico.
World J Microbiol Biotechnol ; 29(2): 301-7, 2013 Feb.
Article en En | MEDLINE | ID: mdl-23054703
ABSTRACT
The dynamism of microbial populations in the rumen has been studied with molecular methods that analyze single nucleotide polymorphisms of ribosomal RNA gene fragments (rDNA). Therefore DNA of good quality is needed for this kind of analysis. In this work we report the evaluation of four DNA extraction protocols (mechanical lysis or chemical lysis with CTAB, ethylxanthogenate or DNAzol(®)) from ruminal fluid. The suitability of two of these protocols (mechanical lysis and DNAzol(®)) was tested on single-strand conformation polymorphism (SSCP) of rDNA of rumen microbial populations. DNAzol(®) was a simple method that rendered good integrity, yield and purity. With this method, subtle changes in protozoan populations were detected in young bulls fed with slightly different formulations of a supplement of multinutritional blocks of molasses and urea. Sequences related to Eudiplodinium maggi and a non-cultured Entodiniomorphid similar to Entodinium caudatum, were related to major fluctuating populations in an SSCP assay.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rumen / Bacterias / ADN Bacteriano / ADN Ribosómico / Métodos Analíticos de la Preparación de la Muestra Tipo de estudio: Evaluation_studies Límite: Animals Idioma: En Revista: World J Microbiol Biotechnol Año: 2013 Tipo del documento: Article País de afiliación: México

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rumen / Bacterias / ADN Bacteriano / ADN Ribosómico / Métodos Analíticos de la Preparación de la Muestra Tipo de estudio: Evaluation_studies Límite: Animals Idioma: En Revista: World J Microbiol Biotechnol Año: 2013 Tipo del documento: Article País de afiliación: México
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