Subcommunity FTIR-spectroscopy to determine physiological cell states.
Curr Opin Biotechnol
; 24(1): 88-94, 2013 Feb.
Article
en En
| MEDLINE
| ID: mdl-23058712
ABSTRACT
Estimation of growth potential in a complex community is a great challenge in biotechnical processes and environmental water quality control. Recently it has been shown that the macromolecular structure is a good indicator for the growth potential of phytoplankton cells. A functional understanding of natural phytoplankton communities requires a community analysis by means of single particles technologies. However, conventional biochemical methods are not sensitive enough to determine the macromolecular composition of a single cell or cell aggregates. This problem can be resolved by Fourier transform infrared (FTIR) spectroscopy, which delivers results similar to biochemical analysis with a much smaller sample size. The combined approach of flow cytometric analysis with subcommunity sorting and subsequent FTIR-analysis offers new perspectives for the understanding of community functioning and process optimization.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fitoplancton
/
Espectroscopía Infrarroja por Transformada de Fourier
Idioma:
En
Revista:
Curr Opin Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Alemania