Detection of Streptococcus pyogenes virulence factors by multiplex PCR.
Virulence
; 3(6): 529-33, 2012 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-23076284
ABSTRACT
Streptococcus pyogenes (GAS) is a human pathogen that causes multiple infections worldwide. The pathogenic properties of GAS strains are often linked to the production of virulence factors such as toxins, proteases or DNases. Detection of virulence factors produced by GAS strains can be used to either determine pathogenic potential of the strain or as a rapid screening and typing method. We recently developed a method to detect simultaneously 20 GAS virulence factors (spd3, sdc, sdaB, sdaD, speB, spyCEP, scpA, mac, sic, speL, K, M, C, I, A, H, G, J, smeZ and ssa) in four low volume multiplex PCR reactions (Borek et al., 2011) and below we present a detailed protocol describing the method.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Streptococcus pyogenes
/
Proteínas Bacterianas
/
Factores de Virulencia
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Virulence
Año:
2012
Tipo del documento:
Article
País de afiliación:
Polonia