Development and validation of automatic HS-SPME with a gas chromatography-ion trap/mass spectrometry method for analysis of volatiles in wines.
Talanta
; 101: 177-86, 2012 Nov 15.
Article
en En
| MEDLINE
| ID: mdl-23158309
ABSTRACT
An automated headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-ion trap/mass spectrometry (GC-IT/MS) was developed in order to quantify a large number of volatile compounds in wines such as alcohols, ester, norisoprenoids and terpenes. The procedures were optimized for SPME fiber selection, pre-incubation temperature and time, extraction temperature and time, and salt addition. A central composite experimental design was used in the optimization of the extraction conditions. The volatile compounds showed optimal extraction using a DVB/CAR/PDMS fiber, incubation of 5 ml of wine with 2g NaCl at 45 °C during 5 min, and subsequent extraction of 30 min at the same temperature. The method allowed the identification of 64 volatile compounds. Afterwards, the method was validated successfully for the most significant compounds and was applied to study the volatile composition of different white wines.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Automatización
/
Vino
/
Microextracción en Fase Sólida
/
Compuestos Orgánicos Volátiles
/
Cromatografía de Gases y Espectrometría de Masas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Talanta
Año:
2012
Tipo del documento:
Article
País de afiliación:
Brasil