Exposure to mono-n-butyl phthalate disrupts the development of preimplantation embryos.
Reprod Fertil Dev
; 25(8): 1174-84, 2013.
Article
en En
| MEDLINE
| ID: mdl-23231764
ABSTRACT
Dibutyl phthalate (DBP), a widely used phthalate, is known to cause many serious diseases, especially in the reproductive system. However, little is known about the effects of its metabolite, mono-n-butyl phthalate (MBP), on preimplantation embryo development. In the present study, we found that treatment of embryos with 10⻳ M MBP impaired developmental competency, whereas exposure to 10â»4 M MBP delayed the progression of preimplantation embryos to the blastocyst stage. Furthermore, reactive oxygen species (ROS) levels in embryos were significantly increased following treatment with 10⻳ M MBP. In addition, 10⻳ M MBP increased apoptosis via the release of cytochrome c, whereas immunofluorescent analysis revealed that exposure of preimplantation embryos to MBP concentration-dependently (10â»5, 10â»4 and 10⻳ M) decreased DNA methylation. Together, the results indicate a possible relationship between MBP exposure and developmental failure in preimplantation embryos.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácidos Ftálicos
/
Solventes
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Blastocisto
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Apoptosis
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Estrés Oxidativo
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Ectogénesis
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Disruptores Endocrinos
Límite:
Animals
Idioma:
En
Revista:
Reprod Fertil Dev
Asunto de la revista:
MEDICINA REPRODUTIVA
Año:
2013
Tipo del documento:
Article
País de afiliación:
China