Converting fluorescence data into Ca2+ concentration.
Cold Spring Harb Protoc
; 2013(2): 126-9, 2013 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-23378651
ABSTRACT
In many situations, fluorescent Ca(2+) reporters are used to simply indicate that a change of Ca(2+) concentration has occurred. Monitoring the emission from a Ca(2+)-sensitive indicator can be sufficient to tell whether a signal has arisen, and what its kinetic/spatial parameters were. The emission from an indicator does not have a linear relationship to the Ca(2+) concentration within a cell; rather, the relationship between fluorescence emission and Ca(2+) concentration is described by a logistic function. Simply recording fluorescence emission, therefore, provides a relative indication of the magnitude of a Ca(2+) signal that should not be used for generating mean amplitude data. However, with a little consideration and effort, the fluorescence output can be calibrated to yield actual Ca(2+) concentration.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría de Fluorescencia
/
Calcio
Idioma:
En
Revista:
Cold Spring Harb Protoc
Año:
2013
Tipo del documento:
Article
País de afiliación:
Reino Unido