ß-galactosidase-catalyzed synthesis of galactosyl chlorphenesin and its characterization.
Appl Biochem Biotechnol
; 171(6): 1299-312, 2013 Nov.
Article
en En
| MEDLINE
| ID: mdl-23564435
We synthesized galactosyl chlorphenesin (CPN-G) using ß-gal-containing Escherichia coli (E. coli) cells in which the conversion yield of chlorphenesin (CPN) to CPN-G reached about 64 % during 12 h. CPN-G was identified and characterized using high-performance liquid chromatography, liquid chromatography-mass spectrometry, Fourier transform-infrared spectrometry, and nuclear magnetic resonance analysis ((1)H and (13)C). We verified that a galactose was covalently bound to a CPN alcohol group during CPN-G synthesis throughout these analyses. In particular, by the hydrolysis of CPN-G using ß-gal, it was confirmed that a galactose was bound to CPN. The minimal inhibitory concentration (MIC) results showed that the CPN-G MICs were fairly similar to those of CPN. HACAT cell viability was significantly higher in CPN-G-treated cells than in CPN-treated cells at concentrations of 0.0-20.0 mM. Finally, we accomplished the synthesis of less toxic CPN-G, compared with CPN, using ß-gal-containing E. coli cells as whole cells without changes in the MICs against microorganisms.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Clorfenesina
/
Beta-Galactosidasa
/
Proteínas de Escherichia coli
/
Escherichia coli
/
Galactosa
Idioma:
En
Revista:
Appl Biochem Biotechnol
Año:
2013
Tipo del documento:
Article