[Identification of a novel HLA allele HLA-B*55:35].

ABSTRACT

OBJECTIVE: To identify a novel human leukocyte antigen (HLA) B allele and explore its family heritage. METHODS: A novel HLA allele was suspected upon routine HLA typing using a polymerase chain reaction-sequence specific oligonucleotide probe (PCR-SSOP) assay. The sequence was confirmed with DNA sequencing and compared with its closest matching allele, B*5502. The family was also investigated. RESULTS: An unusual reaction pattern was detected during routine HLA typing. The sequence was confirmed to be a novel HLA-B allele, which differed from the closest matching allele, B*5502 in 7 nt positions in exon 2. Among the 7 mutations from 6 codons, there were two amino acids changes including 69Glu→Met and 70Glu→Ala. CONCLUSION: A novel HLA-B allele has been identified and officially named as B*5535 by the WHO Nomenclature Committee for Factors of the HLA System (GenBank accession number FJ898284).