Fibroblasts from women with pelvic organ prolapse show differential mechanoresponses depending on surface substrates.
Int Urogynecol J
; 24(9): 1567-75, 2013 Sep.
Article
en En
| MEDLINE
| ID: mdl-23579290
ABSTRACT
INTRODUCTION AND HYPOTHESIS:
Little is known about dynamic cell-matrix interactions in the context of pathophysiology and treatments for pelvic organ prolapse (POP). This study sought to identify differences between fibroblasts from women with varying degrees of prolapse in reaction to mechanical stimuli and matrix substrates in vitro.METHODS:
Fibroblasts from the vaginal wall of three patients with POP Quantification (POP-Q) system stages 0, II, and IV were stretched on artificial polymer substrates either coated or not coated with collagen I. Changes in morphology and anabolic/catabolic compounds that affect matrix remodelling were evaluated at protein- and gene-expression levels. Statistical analysis was performed using one-way analysis of variance (ANOVA), followed by Tukey-Kramer's post hoc test.RESULTS:
POP fibroblasts show delayed cell alignment and lower responses to extracellular matrix remodelling factors at both enzymatic- and gene-expression levels compared with healthy fibroblasts.CONCLUSION:
POP fibroblasts, when compared with healthy cells, show differential mechanoresponses on two artificial polymer substrates. This should be taken into account when designing or improving implants for treating POP.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polímeros
/
Fenómenos Biomecánicos
/
Índice de Severidad de la Enfermedad
/
Uniones Célula-Matriz
/
Prolapso de Órgano Pélvico
/
Fibroblastos
Tipo de estudio:
Prognostic_studies
Límite:
Female
/
Humans
Idioma:
En
Revista:
Int Urogynecol J
Asunto de la revista:
GINECOLOGIA
/
UROLOGIA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Países Bajos