Repeated dose toxicity study of Vibrio cholerae-loaded gastro-resistant microparticles.
J Microencapsul
; 31(1): 86-92, 2014.
Article
en En
| MEDLINE
| ID: mdl-23795905
ABSTRACT
CONTEXT Microencapsulation of antigens has been extensively studied over the last decades aiming at improving the immunogenicity of vaccine candidates. OBJECTIVE:
Addressing microparticles (MPs) toxicity in rats. MATERIAL ANDMETHODS:
Spray-dried Eudragit® L 30 D-55 MPs and Eudragit® L 30 D-55 alginate MPs were elaborated and characterized. MPs obtained were administered to rats, three groups were defined G1, control group; G2, administered with Vibrio cholerae (VC)-loaded MPs; G3, receiving VC-loaded alginate MPs. Animals received three vaccine doses. Body weight, food and water intake were controlled during the study. Haematological parameters, vibriocidal titres, organ weight and histology in necropsy were also analyzed.RESULTS:
All animals grew healthy. Body weight gain, food and water intake and haematological parameters remained within physiological values, showing no treatment-related differences. Moreover, organ weight changes were not detected and animals developed protective vibriocidal titres.CONCLUSION:
VC-loaded MPs and VC-loaded alginate MPs have proved to be safe and effective in the assessed conditions.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
1_ASSA2030
/
2_ODS3
Problema de salud:
1_doencas_transmissiveis
/
2_enfermedades_transmissibles
Asunto principal:
Ácidos Polimetacrílicos
/
Vibrio cholerae
/
Vacunas contra el Cólera
/
Sistemas de Liberación de Medicamentos
Límite:
Animals
Idioma:
En
Revista:
J Microencapsul
Asunto de la revista:
FARMACIA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Cuba