Effect of HMGB1/NF-κB in hyperbaric oxygen treatment on decreasing injury caused by skin flap grafts in rats.
Eur Rev Med Pharmacol Sci
; 17(15): 2010-8, 2013.
Article
en En
| MEDLINE
| ID: mdl-23884820
ABSTRACT
BACKGROUND:
Skin flap grafting (SFG) is a common surgical operation, and hyperbaric oxygen treatment (HBOT) is an important strategy for restoring the grafted skin flap. Thus, we employed a rat skin flap grafting model treated with HBO, and expression levels of high mobility group protein 1 (HMGB1) and NF-kappaB (nuclear factor-kappaB) were characterized. MATERIALS ANDMETHODS:
Forty rats were randomly assigned to 5 groups (1) sham-operation (SH), (2) ischemia followed by reperfusion 3 days after operation (IR3d), (3) ischemia followed by reperfusion 5 days after operation (IR5d), (4) ischemia followed by reperfusion and HBOT 3 days after operation (HBO3d), and (5) ischemia followed by reperfusion and HBOT 5 days after operation (HBO5d). Elevated pedicled skin flaps were designed (size, 9 cm × 6 cm), and feeding vessels were clamped. The microvascular clamp was removed 3 h later and flow was restored. In the HBO3d and HBO5d groups, rats received 1 h of hyperbaric oxygen (HBO) starting immediately after surgery for 3 days and 5 days, respectively. Upon completion of animal experiments, rats were euthanized by general anesthesia, and blood samples were taken for testing. The tissues were sectioned for western blotting and immunohistochemical staining.RESULTS:
Expression of HMGB1 and NF-κB proteins in the HBO groups was lower than in the IR groups.CONCLUSIONS:
The results suggest that HBOT can be used to reduce ischemia-reperfusion (IR) injury of skin flap grafts.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Piel
/
Daño por Reperfusión
/
FN-kappa B
/
Proteína HMGB1
/
Oxigenoterapia Hiperbárica
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Eur Rev Med Pharmacol Sci
Asunto de la revista:
FARMACOLOGIA
/
TOXICOLOGIA
Año:
2013
Tipo del documento:
Article