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Decreased expression of surfactant protein genes is associated with an increased expression of Forkhead box M1 gene in the fetal lung tissues of premature rabbits.
Hahn, Won-Ho; Chang, Ji-Young; Lee, Kyung Suk; Bae, Chong-Woo.
Afiliación
  • Hahn WH; Department of Pediatrics, Kyung Hee University Hospital at Gangdong, 892 Dongnam-ro, Gangdong-gu, Seoul 134-727, Korea. baecw@khnmc.or.kr.
Yonsei Med J ; 54(6): 1422-9, 2013 Nov.
Article en En | MEDLINE | ID: mdl-24142647
ABSTRACT

PURPOSE:

Recently, Forkhead box M1 (FoxM1) was reported to be correlated with lung maturation and expression of surfactant proteins (SPs) in mice models. However, no study has been conducted in rabbit lungs despite their high homology with human lungs. Thus, we attempted to investigate serial changes in the expressions of FoxM1 and SP-A/B throughout lung maturation in rabbit fetuses. MATERIALS AND

METHODS:

Pregnant New Zealand White rabbits were grouped according to gestational age from 5 days before to 2 days after the day of expected full term delivery (F5, F4, F3, F2, F1, F0, P1, and P2). A total of 64 fetuses were enrolled after Cesarean sections. The expressions of mRNA and proteins of FoxM1 and SP-A/B in fetal lung tissue were tested by quantitative reverse-transcriptase real-time PCR and Western blot. Furthermore, their correlations were analyzed.

RESULTS:

The mRNA expression of SP-A/B showed an increasing tendency positively correlated with gestational age, while the expression of FoxM1 mRNA and protein decreased from F5 to F0. A significant negative correlation was found between the expression levels of FoxM1 and SP-A/B (SP-A R=-0.517, p=0.001; SP-B R=-0.615, p<0.001).

CONCLUSION:

Preterm rabbits demonstrated high expression of FoxM1 mRNA and protein in the lungs compared to full term rabbits. Also, the expression of SP-A/B was inversely related with serial changes in FoxM1 expression. This is the first report to suggest an association between FoxM1 and expression of SP-A/B and lung maturation in preterm rabbits.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteína A Asociada a Surfactante Pulmonar / Factores de Transcripción Forkhead / Feto / Pulmón Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals / Pregnancy Idioma: En Revista: Yonsei Med J Año: 2013 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteína A Asociada a Surfactante Pulmonar / Factores de Transcripción Forkhead / Feto / Pulmón Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals / Pregnancy Idioma: En Revista: Yonsei Med J Año: 2013 Tipo del documento: Article
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