Easy determination of ploidy level in Arabidopsis thaliana plants by means of pollen size measurement.
Plant Cell Rep
; 13(11): 652-6, 1994 Aug.
Article
en En
| MEDLINE
| ID: mdl-24196247
ABSTRACT
Cytogenetic examination of transgenic Arabidopsis thaliana (L.) Heynh. plants obtained by Agrobacterium-mediated gene transfer to cotyledon- and root-explants or by direct gene transfer into protoplasts revealed a high percentage of tetraploid or aneuploid transformants. Depending on the transformation procedure used, 13% (root explant transformation), 33% (cotyledon explant transformation), or 38% (direct gene transfer) of the transformants showed aberrant ploidy levels. A good correlation between the ploidy level of a plant and the size of its pollen grains was observed. This allows quick and simple testing of the ploidy level of transgenic Arabidopsis plants.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Plant Cell Rep
Asunto de la revista:
BOTANICA
Año:
1994
Tipo del documento:
Article
País de afiliación:
Alemania