Comprehensive multiphase NMR spectroscopy of intact ¹³C-labeled seeds.
J Agric Food Chem
; 62(1): 107-15, 2014 Jan 08.
Article
en En
| MEDLINE
| ID: mdl-24354469
ABSTRACT
Seeds are complex entities composed of liquids, gels, and solids. NMR spectroscopy is a powerful tool for studying molecular structure but has evolved into two fields, solution and solid state. Comprehensive multiphase (CMP) NMR spectroscopy is capable of liquid-, gel-, and solid-state experiments for studying intact samples where all organic components are studied and differentiated in situ. Herein, intact (13)C-labeled seeds were studied by a variety of 1D/2D (1)H/(13)C experiments. In the mobile phase, an assortment of metabolites in a single (13)C-labeled wheat seed were identified; the gel phase was dominated by triacylglycerides; the semisolid phase was composed largely of carbohydrate biopolymers, and the solid phase was greatly influenced by starchy endosperm signals. Subsequently, the seeds were compared and relative similarities and differences between seed types discussed. This study represents the first application of CMP-NMR to food chemistry and demonstrates its general utility and feasibility for studying intact heterogeneous samples.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Semillas
/
Espectroscopía de Resonancia Magnética
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
J Agric Food Chem
Año:
2014
Tipo del documento:
Article