Microbicidal power of alpha radiation in sterilizing germinating Bacillus anthracis spores.
Antimicrob Agents Chemother
; 58(3): 1813-5, 2014.
Article
en En
| MEDLINE
| ID: mdl-24379209
ABSTRACT
Radioimmunotherapy (RIT) takes advantage of the specificity and affinity of the antigen-antibody interaction to deliver microbicidal radioactive nuclides to a site of infection. In this study, we investigated the microbicidal properties of an alpha particle-emitting 213Bi-labeled monoclonal antibody (MAb), EA2-1 (213Bi-EA2-1), that binds to the immunodominant antigen on Bacillus anthracis spores. Our results showed that dormant spores were resistant to 213Bi-EA2-1. Significant spore killing was observed following treatment with EA2-1 labeled with 300 µCi 213Bi; however, this effect was not dependent on the MAb. In contrast, when spores were germinating, 213Bi-EA2-1 mediated MAb-specific killing in a dose-dependent manner. Dormant spores are very resistant to RIT, and RIT should focus on targeting vegetative cells and germinating spores.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Esporas Bacterianas
/
Bacillus anthracis
/
Partículas alfa
Idioma:
En
Revista:
Antimicrob Agents Chemother
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos