Role of soybean ß-conglycinin subunits as potential dietary allergens in piglets.
Vet J
; 199(3): 434-8, 2014 Mar.
Article
en En
| MEDLINE
| ID: mdl-24508322
ABSTRACT
ß-Conglycinin, a major seed-storage protein in soybeans, is one of the primary antigenic proteins responsible for soybean-meal hypersensitivity in weaned piglets. The protein is a heterotrimer composed of subunits α, α' and ß. It is currently unknown which of the ß-conglycinin subunits are allergenic for piglets. The aim of this study was to identify potential allergenic subunits of ß-conglycinin for soybean sensitive piglets and to characterise these subunits by immunoglobulin (Ig) G and E immunoblotting, ELISA, 'skin prick' and whole blood histamine-release testing. The IgG and IgE binding capabilities of the purified α, α' and ß subunits of ß-conglycinin were determined by immunoblot analysis and ELISA with sera from ß-conglycinin sensitised piglets. Skin prick testing and whole blood histamine release testing were also performed to detect the activated effector cell response to specific allergens. Specific IgG and E antibodies were identified that recognised all three subunits of ß-conglycinin in the sera of ß-conglycinin sensitised piglets. All three subunits of ß-conglycinin elicited positive skin test and specific histamine release responses from the whole blood of ß-conglycinin sensitised piglets. These results suggest that all three ß-conglycinin subunits are potential allergens for piglets.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Glycine max
/
Porcinos
/
Alérgenos
/
Proteínas de Soja
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Antígenos de Plantas
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Proteínas de Almacenamiento de Semillas
/
Globulinas
Límite:
Animals
Idioma:
En
Revista:
Vet J
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2014
Tipo del documento:
Article
País de afiliación:
China