The DR6 protein from human herpesvirus-6B induces p53-independent cell cycle arrest in G2/M.
Virology
; 452-453: 254-63, 2014 Mar.
Article
en En
| MEDLINE
| ID: mdl-24606703
ABSTRACT
HHV-6B infection inhibits cell proliferation in G2/M, but no protein has so far been recognized to exert this function. Here we identify the protein product of direct repeat 6, DR6, as an inhibitor of G2/M cell-cycle progression. Transfection of DR6 reduced the total number of cells compared with mock-transfected cells. Lentiviral transduction of DR6 inhibited host cell DNA synthesis in a p53-independent manner, and this inhibition was DR6 dose-dependent. A deletion of 66 amino acids from the N-terminal part of DR6 prevented efficient nuclear translocation and the ability to inhibit DNA synthesis. DR6-induced accumulation of cells in G2/M was accompanied by an enhanced expression of cyclin B1 that accumulated predominantly in the cytoplasm. Pull-down of cyclin B1 brought down pCdk1 with the inactivating phosphorylation at Tyr15. Together, DR6 delays cell cycle with an accumulation of cells in G2/M and thus might be involved in HHV-6B-induced cell-cycle arrest.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Virales
/
Proteína p53 Supresora de Tumor
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Herpesvirus Humano 6
/
Infecciones por Roseolovirus
/
Puntos de Control de la Fase G2 del Ciclo Celular
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Puntos de Control de la Fase M del Ciclo Celular
Límite:
Humans
Idioma:
En
Revista:
Virology
Año:
2014
Tipo del documento:
Article
País de afiliación:
Dinamarca