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Cryogenic transmission electron microscopy of recombinant tuberculosis vaccine antigen with anionic liposomes reveals formation of flattened liposomes.
Fox, Christopher B; Mulligan, Sean K; Sung, Joyce; Dowling, Quinton M; Fung, H W Millie; Vedvick, Thomas S; Coler, Rhea N.
Afiliación
  • Fox CB; Infectious Disease Research Institute, Seattle, WA, USA.
  • Mulligan SK; NanoImaging Services, La Jolla, CA, USA.
  • Sung J; NanoImaging Services, La Jolla, CA, USA.
  • Dowling QM; Infectious Disease Research Institute, Seattle, WA, USA.
  • Fung HW; Infectious Disease Research Institute, Seattle, WA, USA.
  • Vedvick TS; Infectious Disease Research Institute, Seattle, WA, USA.
  • Coler RN; Infectious Disease Research Institute, Seattle, WA, USA.
Int J Nanomedicine ; 9: 1367-77, 2014.
Article en En | MEDLINE | ID: mdl-24648734
Development of lipid-based adjuvant formulations to enhance the immunogenicity of recombinant vaccine antigens is a focus of modern vaccine research. Characterizing interactions between vaccine antigens and formulation excipients is important for establishing compatibility between the different components and optimizing vaccine stability and potency. Cryogenic transmission electron microscopy (TEM) is a highly informative analytical technique that may elucidate various aspects of protein- and lipid-based structures, including morphology, size, shape, and phase structure, while avoiding artifacts associated with staining-based TEM. In this work, cryogenic TEM is employed to characterize a recombinant tuberculosis vaccine antigen, an anionic liposome formulation, and antigen-liposome interactions. By performing three-dimensional tomographic reconstruction analysis, the formation of a population of protein-containing flattened liposomes, not present in the control samples, was detected. It is shown that cryogenic TEM provides unique information regarding antigen-liposome interactions not detectable by light-scattering-based methods. Employing a suite of complementary analytical techniques is important to fully characterize interactions between vaccine components.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 1_ASSA2030 / 2_ODS3 / 3_ND Problema de salud: 1_doencas_transmissiveis / 2_enfermedades_transmissibles / 3_neglected_diseases / 3_tuberculosis Asunto principal: Vacunas contra la Tuberculosis / Antígenos Bacterianos Límite: Animals / Humans Idioma: En Revista: Int J Nanomedicine Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 1_ASSA2030 / 2_ODS3 / 3_ND Problema de salud: 1_doencas_transmissiveis / 2_enfermedades_transmissibles / 3_neglected_diseases / 3_tuberculosis Asunto principal: Vacunas contra la Tuberculosis / Antígenos Bacterianos Límite: Animals / Humans Idioma: En Revista: Int J Nanomedicine Año: 2014 Tipo del documento: Article País de afiliación: Estados Unidos
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