Antibodies against an alpha-bungarotoxin-binding peptide of the alpha-subunit of the acetylcholine receptor.
Biochem Biophys Res Commun
; 160(1): 289-95, 1989 Apr 14.
Article
en En
| MEDLINE
| ID: mdl-2469418
ABSTRACT
Polyclonal and monoclonal antibodies were raised against a peptide comprising residues 173-204 of the alpha-subunit of the acetylcholine receptor. The polyclonal and pooled monoclonal antibodies inhibited up to 50% of 125I-alpha-bungarotoxin binding to peptide 173-204. Some of the antibodies recognized native receptor but did not significantly affect alpha-bungarotoxin binding. Epitope mapping revealed that the antibodies are directed against residues 183-194 indicating this region is a major determinant of toxin binding. This region is most likely conformationally constrained in the native receptor.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fragmentos de Péptidos
/
Bungarotoxinas
/
Receptores Colinérgicos
/
Epítopos
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1989
Tipo del documento:
Article