Coexpression of multiple genes reconstitutes two pathways of very long-chain polyunsaturated fatty acid biosynthesis in Pichia pastoris.
Biotechnol Lett
; 36(9): 1843-51, 2014 Sep.
Article
en En
| MEDLINE
| ID: mdl-24863294
ABSTRACT
The introduction of novel traits to cells often requires the stable coexpression of multiple genes within the same cell. Herein, we report that C22 very long-chain polyunsaturated fatty acids (VLC-PUFAs) were synthesized from C18 precursors by reactions catalyzed by delta 6-desaturase, an ELOVL5 involved in VLC-PUFA elongation, and delta 5-desaturase. The coexpression of McD6DES, AsELOVL5, and PtD5DES encoding the corresponding enzymes, produced docosatetraenoic acid (C224 n-6) and docosapentaenoic acid (C225 n-3), as well as arachidonic acid (C204 n-6) and eicosapentaenoic acid (C205 n-3) in the methylotrophic yeast Pichia pastoris. The expression of each gene increased within 24 h, with high transcript levels after induction with 0.5 or 1 % methanol. High levels of the newly expressed VLC-PUFAs occurred after 144 h. This expression system exemplifies the recent progress and future possibilities of the metabolic engineering of VLC-PUFAs in oilseed crops.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pichia
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Expresión Génica
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Vías Biosintéticas
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Ácidos Grasos Insaturados
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Ingeniería Metabólica
Idioma:
En
Revista:
Biotechnol Lett
Año:
2014
Tipo del documento:
Article