A novel fluid resuscitation protocol: provide more protection on acute kidney injury during septic shock in rats.

ABSTRACT

OBJECTIVE: To investigate the therapeutic effects of a novel fluid resuscitation protocol (early fluid resuscitation plus 2% hydrogen inhalation) on acute kidney injury during septic shock induced by lipopolysaccharide in rats. METHODS: Sixty male Wistar rats were randomly divided into four groups (n = 15 per group) control group (C), septic shock group (S), septic shock with early fluid resuscitation group (R), and septic shock with early fluid resuscitation plus 2% hydrogen inhalation group (R+R+H2). The rats were ventilated, and a 2% hydrogen mixture was used in Group R+H2. Lipopolysaccharide (10 mg/kg) was administered to establish the septic shock model in rats and fluid resuscitation was performed in Groups R and R+R+H2. RESULTS: Fluid resuscitation with 2% hydrogen inhalation decreased serum creatinine, blood urea nitrogen, and neutrophil gelatinase-associated lipocalin. It also reduced oxidative stress injury and decreased renal tumor necrosis factor-α and interleukin-6 levels compared with fluid resuscitation alone. CONCLUSION: Early fluid resuscitation plus 2% hydrogen inhalation provided more protection against AKI during septic shock.