Isolation and mRNA expression of hypoxia-inducible factor α (HIF-α) in two sublittoral nassariid gastropods: Nassarius siquijorensis and Nassarius conoidalis.

ABSTRACT

Hypoxia-inducible factor (HIF) is commonly found in vertebrates as an adaptation against hypoxia. In this study, partial sequences of HIF were first reported for subtidal nassariid gastropods, Nassarius siquijorensis and Nassarius conoidalis. The phylogenetic tree of HIF-α among various animal groups was inferred. Adults of both N. siquijorensis and N. conoidalis were exposed to 24 h and 1 week hypoxia (1.5 mg O2 l(-1)) and the change of HIF-α mRNA level was determined in gill, foot muscle and hepatopancreas by using quantitative real-time PCR. The expression profile of HIF-α was also investigated in veliger larvae after 1 and 24 h hypoxia exposure. The HIF-α mRNA from gills of N. siquijorensis was 2.22-fold elevated after 24 h of exposure but returned back to the baseline level after 1 week of exposure. In contrast to N. siquijorensis, both short-term and long-term exposure to hypoxia significantly enhanced the mRNA level of HIF-α in the gill of N. conoidalis. Both 24 h and 1 week of exposure resulted in a significantly higher degree of expression of mRNA of HIF-α in the foot muscle of N. siquijorensis and N. conoidalis. For hepatopancreas, the mRNA level of HIF-α remained unchanged in N. siquijorensis after both 24 h and 1 week of exposure to hypoxia but a significant enhancement was observed in N. conoidalis after 1 week of exposure to hypoxia. The mRNA level of HIF-α significantly reduced after 24 h of exposure to hypoxia in the larvae of N. siquijorensis. The different expression patterns of HIF-α mRNA between the two species reflected their different adaptation abilities to hypoxia, which will further explain the differences in the distribution of these species in Hong Kong waters with N. siquijorensis being predominant in regions suffered from summer hypoxia.