High-speed force mapping on living cells with a small cantilever atomic force microscope.
Rev Sci Instrum
; 85(7): 073703, 2014 Jul.
Article
en En
| MEDLINE
| ID: mdl-25085142
ABSTRACT
The imaging speed of the wide-spread force mapping mode for quantitative mechanical measurements on soft samples in liquid with the atomic force microscope (AFM) is limited by the bandwidth of the z-scanner and viscous drag forces on the cantilever. Here, we applied high-speed, large scan-range atomic force microscopy and small cantilevers to increase the speed of force mapping by ≈10-100 times. This allowed resolving dynamic processes on living mouse embryonic fibroblasts. Cytoskeleton reorganization during cell locomotion, growth of individual cytoskeleton fibers, cell blebbing, and the formation of endocytic pits in the cell membrane were observed. Increasing the force curve rate from 2 to 300 Hz increased the measured apparent Young's modulus of the cells by about 10 times, which facilitated force mapping measurements at high speed.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microscopía de Fuerza Atómica
Límite:
Animals
Idioma:
En
Revista:
Rev Sci Instrum
Año:
2014
Tipo del documento:
Article
País de afiliación:
Alemania