Your browser doesn't support javascript.
loading
Tracheal tissue engineering in rats.
Jungebluth, Philipp; Haag, Johannes C; Sjöqvist, Sebastian; Gustafsson, Ylva; Beltrán Rodríguez, Antonio; Del Gaudio, Costantino; Bianco, Alessandra; Dehnisch, Ivar; Uhlén, Per; Baiguera, Silvia; Lemon, Greg; Lim, Mei Ling; Macchiarini, Paolo.
Afiliación
  • Jungebluth P; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
  • Haag JC; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
  • Sjöqvist S; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
  • Gustafsson Y; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
  • Beltrán Rodríguez A; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
  • Del Gaudio C; Department of Enterprise Engineering, University of Rome, Intrauniversity Consortium for Material Science and Technology (INSTM), Research Unit 'Tor Vergata', Rome, Italy.
  • Bianco A; Department of Enterprise Engineering, University of Rome, Intrauniversity Consortium for Material Science and Technology (INSTM), Research Unit 'Tor Vergata', Rome, Italy.
  • Dehnisch I; Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
  • Uhlén P; Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
  • Baiguera S; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
  • Lemon G; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
  • Lim ML; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
  • Macchiarini P; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
Nat Protoc ; 9(9): 2164-79, 2014 Sep.
Article en En | MEDLINE | ID: mdl-25122525
Tissue-engineered tracheal transplants have been successfully performed clinically. However, before becoming a routine clinical procedure, further preclinical studies are necessary to determine the underlying mechanisms of in situ tissue regeneration. Here we describe a protocol using a tissue engineering strategy and orthotopic transplantation of either natural decellularized donor tracheae or artificial electrospun nanofiber scaffolds into a rat model. The protocol includes details regarding how to assess the scaffolds' biomechanical properties and cell viability before implantation. It is a reliable and reproducible model that can be used to investigate the crucial aspects and pathways of in situ tracheal tissue restoration and regeneration. The model can be established in <6 months, and it may also provide a means to investigate cell-surface interactions, cell differentiation and stem cell fate.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tráquea / Ingeniería de Tejidos / Regeneración Tisular Dirigida / Andamios del Tejido Tipo de estudio: Guideline Límite: Animals Idioma: En Revista: Nat Protoc Año: 2014 Tipo del documento: Article País de afiliación: Suecia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tráquea / Ingeniería de Tejidos / Regeneración Tisular Dirigida / Andamios del Tejido Tipo de estudio: Guideline Límite: Animals Idioma: En Revista: Nat Protoc Año: 2014 Tipo del documento: Article País de afiliación: Suecia