A calix[2]triazole[2]arene-based fluorescent chemosensor for probing the copper trafficking pathway in Wilson's disease.
Dalton Trans
; 43(43): 16178-82, 2014 Nov 21.
Article
en En
| MEDLINE
| ID: mdl-25260151
ABSTRACT
A pyrenyl-appended calix[2]triazole[2]arene displaying excellent selectivity for Cu(2+) over other metal ions in pyrenyl excimer emission changes was synthesized. The binding mode with Cu(2+) was supported through nuclear magnetic resonance (NMR) studies and density functional theory (DFT) calculations. Fluorescence imaging demonstrates that this new copper sensor is capable of detecting intracellular copper in living cells. Furthermore, through colocalization of the probe with organelle trackers as a function of time, it was observed that copper initially accumulates in lysosomes and then decreases. These results provide evidence for a close relationship between copper and lysosomes in Wilson's disease. This system is the first Cu(2+) ion-induced fluorescent turn-on system used for imaging copper trafficking over time in a Wilson's disease model.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cobre
/
Calixarenos
/
Colorantes Fluorescentes
/
Degeneración Hepatolenticular
/
Microscopía Fluorescente
Límite:
Humans
Idioma:
En
Revista:
Dalton Trans
Asunto de la revista:
QUIMICA
Año:
2014
Tipo del documento:
Article