Isolation and genetic analysis of an environmental bacteriophage: A 10-session laboratory series in molecular virology.
Biochem Mol Biol Educ
; 42(6): 480-5, 2014.
Article
en En
| MEDLINE
| ID: mdl-25331656
ABSTRACT
Bacterial viruses, otherwise known as bacteriophage (or phage), are some of the most abundant viruses found in the environment. They can be easily isolated from water or soil and are ideal for use in laboratory classrooms due to their ease of culture and inherent safety. Here, we describe a series of 10 laboratory exercises where students collect, isolate, and purify the genome of an environmental phage. Once the genome has been extracted, students then clone a fragment of their isolated phage genome into a plasmid and analyze its sequence to identify the phage in their original isolate. These exercises have been carefully designed to apply foundational concepts that will expose students to basic skills in microbiology, molecular biology, and bioinformatics.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacteriófagos
/
Virología
/
Curriculum
/
Biología Molecular
Idioma:
En
Revista:
Biochem Mol Biol Educ
Año:
2014
Tipo del documento:
Article