Evaluation of ultrasmall superparamagnetic iron oxide contrast agent labeling of equine cord blood and bone marrow mesenchymal stromal cells.
Am J Vet Res
; 75(11): 1010-7, 2014 Nov.
Article
en En
| MEDLINE
| ID: mdl-25350092
ABSTRACT
OBJECTIVE:
To evaluate the efficacy and effects of labeling equine umbilical cord blood (UCB)- and bone marrow (BM)-derived multipotent mesenchymal stromal cells (MSCs) with an ultrasmall superparamagnetic iron oxide (SPIO) contrast agent and the detection of labeled MSCs by use of MRI. SAMPLE UCB MSCs from placental tissues of 5 foals and BM MSCs from 5 horses. PROCEDURES UCB and BM MSC cultures were seeded in duplicate (5,000 cells/cm(2)). One duplicate was incubated with SPIO (50 µg/mL); the other was processed identically, but without SPIO. Mesenchymal stromal cells were expanded in triplicates for 5 passages and assessed for viability and proliferative capacity, labeling efficacy, and labeled cell proportion. For MRI detection, 5 × 10(6) labeled BM MSCs from passage 1 or 2 were injected into a collagenase-induced superficial digital flexor tendon defect of an equine cadaveric forelimb from 2 horses.RESULTS:
For passages 1, 2, and 3, labeling efficacy and cell proportion for UCB MSCs (99.6% [range, 98.8% to 99.9%], 16.6% [range, 6.5% to 36.1%], and 1.0% [range, 0.4% to 2.8%], respectively) were significantly higher than for BM MSCs (99.2% [range, 97.8% to 99.7%], 4.5% [range, 1.6% to 11.8%], and 0.2% [range, 0.1% to 0.6%], respectively). Labeling was not detectable after passage 3. Viability of MSCs was not affected, but cell doubling time increased in labeled MSCs, compared with that of unlabeled MSCs. On MRI 3-D T2*-weighted fast gradient echo sequences, decreased signal intensity was observed for BM passage 1 MSCs. CONCLUSIONS AND CLINICAL RELEVANCE Equine UCB and BM MSCs were labeled with SPIO at high efficiencies.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Dextranos
/
Medios de Contraste
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Nanopartículas de Magnetita
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Sangre Fetal
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Células Madre Mesenquimatosas
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Caballos
Límite:
Animals
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Pregnancy
Idioma:
En
Revista:
Am J Vet Res
Año:
2014
Tipo del documento:
Article
País de afiliación:
Canadá