Determination of antipyrine in saliva using the dispersive liquid-liquid microextraction based on a stepwise injection system.
Talanta
; 133: 66-70, 2015 Feb.
Article
en En
| MEDLINE
| ID: mdl-25435228
ABSTRACT
A fully automated stepwise injection spectrophotometric method for determination of antipyrine in saliva (agent for non-invasive assessment of the activity of the drug metabolizing system in hepatocytes) has been developed. The method is based on the antipyrine derivatization by nitrite-ion dispersive liquid-liquid microextraction (DLLME) of formed 4-nitrosoantipyrine with subsequent UV-vis spectrophotometric detection. Under optimal experimental conditions (0.5 Ð sulfuric acid, 6×10(-3) Ð sodium nitrite, time 6 min) the absorbance of the colored extract at the 345 nm obeys Beer׳s law in the range of 3-200 µM of antipyrine in saliva. The LOD, calculated from a blank test, based on 3σ, found to be 1 µM. The relative standard deviation for the determination of 50 µM antipyrine was 4.5% (n=10). The proposed method was successfully applied to the determination of antipyrine in saliva and the analytical results agreed fairly well with the results obtained by reference HPLC method.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saliva
/
Antiinflamatorios no Esteroideos
/
Antipirina
/
Microextracción en Fase Líquida
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
Talanta
Año:
2015
Tipo del documento:
Article
País de afiliación:
Rusia