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Building the Connectivity Map of epigenetics: chromatin profiling by quantitative targeted mass spectrometry.
Creech, Amanda L; Taylor, Jordan E; Maier, Verena K; Wu, Xiaoyun; Feeney, Caitlin M; Udeshi, Namrata D; Peach, Sally E; Boehm, Jesse S; Lee, Jeannie T; Carr, Steven A; Jaffe, Jacob D.
Afiliación
  • Creech AL; The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
  • Taylor JE; The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
  • Maier VK; Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, United States.
  • Wu X; The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
  • Feeney CM; The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
  • Udeshi ND; The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
  • Peach SE; The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
  • Boehm JS; The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
  • Lee JT; Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, United States; Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02114, United States.
  • Carr SA; The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States.
  • Jaffe JD; The Broad Institute of MIT and Harvard University, 415 Main St., Cambridge, MA 02142, United States. Electronic address: jjaffe@broadinstitute.org.
Methods ; 72: 57-64, 2015 Jan 15.
Article en En | MEDLINE | ID: mdl-25448295
ABSTRACT
Epigenetic control of genome function is an important regulatory mechanism in diverse processes such as lineage commitment and environmental sensing, and in disease etiologies ranging from neuropsychiatric disorders to cancer. Here we report a robust, high-throughput targeted, quantitative mass spectrometry (MS) method to rapidly profile modifications of the core histones of chromatin that compose the epigenetic landscape, enabling comparisons among cells with differing genetic backgrounds, genomic perturbations, and drug treatments.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas / Cromatina / Epigenómica Límite: Humans Idioma: En Revista: Methods Asunto de la revista: BIOQUIMICA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas / Cromatina / Epigenómica Límite: Humans Idioma: En Revista: Methods Asunto de la revista: BIOQUIMICA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos