Your browser doesn't support javascript.
loading
[Individual variability of immunological markers, radiosensitivity and oxidative status in blood lymphocytes of Moscow residents].
Radiats Biol Radioecol ; 53(6): 567-74, 2013.
Article en Ru | MEDLINE | ID: mdl-25486738
ABSTRACT
Expression of activation (CD69) and proliferation (Ki67) markers, their connection with each other, with the oxidative status (reactive oxygen species--ROS) and with radiosensitivity (determined by micronucleus test) have been studied on stimulated blood lymphocytes from Moscow inhabitants. It was shown that the content of T-lymphocytes with the expressed CD69 and the content of T-lymphocytes with the expressed Ki67 markers correlate (r = 0.571; p = 0.0004). We can suppose that expression of the CD69 marker (24 h after PHA stimulation) is needed for the cell cycle progression, but it is not enough for the high expression of Ki67 markers 48 h after stimulation (DNA synthesis phase). It was discovered that T-lymphocytes with the CD69 marker or T-lymphocytes with the Ki67 marker are connected by the negative correlation with the frequency of irradiated cell with micronucleus (MN) r = -0.487; p = 0.010; r = -0.440; p = 0.008, respectively. So we can suppose that lymphocyte radiosensitivity decreased with the increase of expression activation and proliferation markers. It was shown that radiosensitivity determined by MN test is not connected with the oxidative status determined by the reactive oxygen species content including superoxide anion radicals. It is possible to explain by the fact that the ROS concentration has been determined in non-stimulated lymphocytes, but frequencies of cells with MN - in the stimulated cells 48 h after stimulation. Using separate analysis of individual differences by the studied parameters that were determined in the same people, it was shown that individual differences are high enough in the same cases. For example, the radiosensitivity when cells were irradiated 48 h after stimulation, ROS concentration, cell content with activation and proliferation markers. In conclusion, we can say that we failed to find important correlation between the parameters studied. However, the presence of individual differences in the marker expression, the frequency of MN cells, the oxidative status in the usual inhabitants, typical donors in Moscow, is very important.
Asunto(s)
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T / Antígenos de Diferenciación de Linfocitos T / Antígenos CD / Estrés Oxidativo / Antígeno Ki-67 / Lectinas Tipo C Límite: Humans País/Región como asunto: Europa Idioma: Ru Revista: Radiats Biol Radioecol Asunto de la revista: GENETICA / MEDICINA NUCLEAR Año: 2013 Tipo del documento: Article
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T / Antígenos de Diferenciación de Linfocitos T / Antígenos CD / Estrés Oxidativo / Antígeno Ki-67 / Lectinas Tipo C Límite: Humans País/Región como asunto: Europa Idioma: Ru Revista: Radiats Biol Radioecol Asunto de la revista: GENETICA / MEDICINA NUCLEAR Año: 2013 Tipo del documento: Article
...