Effects of microwaves (900 MHz) on peroxidase systems: A comparison between lactoperoxidase and horseradish peroxidase.

Barteri, Mario; De Carolis, Roberta; Marinelli, Fiorenzo; Tomassetti, Goliardo; Montemiglio, Linda Celeste

Barteri, Mario; De Carolis, Roberta; Marinelli, Fiorenzo; Tomassetti, Goliardo; Montemiglio, Linda Celeste.

Afiliación

Barteri M; a Department of Chemistry , "Sapienza" University of Rome , Rome , Italy.
De Carolis R; b National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA) , Rome , Italy.
Marinelli F; c National Research Council of Italy (CNR), Instituto di Genetica Molecolare (IGM) , Bologna , Italy.
Tomassetti G; d SC Laboratory of Muscoloskeletal Cell Biology, Istituto Ortopedico Rizzoli (IOR) , Bologna , Italy.
Montemiglio LC; e Institute of Radioastronomy, Italian National Institute for Astrophysics (INAF) , Bologna , Italy.

Electromagn Biol Med ; 35(2): 126-33, 2016.

Article en En | MEDLINE | ID: mdl-25577980

RESUMEN

This work shows the effects of exposure to an electromagnetic field at 900 MHz on the catalytic activity of the enzymes lactoperoxidase (LPO) and horseradish peroxidase (HRP). Experimental evidence that irradiation causes conformational changes of the active sites and influences the formation and stability of the intermediate free radicals is documented by measurements of enzyme kinetics, circular dichroism spectroscopy (CD) and cyclic voltammetry.

Asunto(s)

Peroxidasa de Rábano Silvestre/metabolismo; Lactoperoxidasa/metabolismo; Microondas/efectos adversos; Dominio Catalítico/efectos de la radiación; Peroxidasa de Rábano Silvestre/química; Cinética; Lactoperoxidasa/química

Palabras clave

900 MHz; electromagnetic fields; enzyme kinetics; horseradish peroxidase; lactoperoxidase; microwaves; radiofrequency

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Peroxidasa de Rábano Silvestre / Lactoperoxidasa / Microondas Idioma: En Revista: Electromagn Biol Med Asunto de la revista: BIOLOGIA / FISIOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Italia

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