[Digital PCR compartmentalization I. Single-molecule detection of rare mutations]. / PCR digitale en micro-compartiments - I. Détection sensible de séquences d'acides nucléiques rares.
Med Sci (Paris)
; 31(1): 84-92, 2015 Jan.
Article
en Fr
| MEDLINE
| ID: mdl-25658735
ABSTRACT
Polymerase chain reaction based techniques have been widely used in laboratory settings. Several applications in oncology, virology or prenatal diagnosis require highly sensitive detection methods, which cannot be achieved with conventional techniques. Digital PCR (dPCR) was developed from the association of PCR and limiting dilution procedures. It is based on the compartmentalization of DNA molecules in small volumes. Controlling the size and the content of each compartment is crucial to obtain a high sensitivity with a single molecule resolution. Microfluidics offers promising tools to isolate DNA fragments such as microdroplets, microchambers or microwells with volumes ranging from few picoliters to nanoliters. The review provides an overview of recent developments of microfluidics dPCR platforms and how this technology can influence the management of cancer patients.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Análisis Mutacional de ADN
/
Reacción en Cadena de la Polimerasa
/
Técnicas de Diagnóstico Molecular
/
Microfluídica
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
Fr
Revista:
Med Sci (Paris)
Asunto de la revista:
MEDICINA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Francia