Genomic and proteomic features of mycobacteriophage SWU1 isolated from China soil.
Gene
; 561(1): 45-53, 2015 Apr 25.
Article
en En
| MEDLINE
| ID: mdl-25701596
ABSTRACT
Mycobacteriophage SWU1 is a newly isolated phage from soil sample collected in Sichuan province, China using Mycobacterium smegmatis mc(2)155 as host. Plaque, phage morphology and one-step growth curve were characterized. The complete genomic sequence of phage SWU1 was determined by shotgun sequencing. The ends of SWU1 were determined. Structural proteins of SWU1 were analyzed by NanoLC-ESI-MS/MS. Seven ORFs were identified as structural protein encoded by SWU1 genome. The genetic basis underlying the SWU1 plaque was explored using comparative genomics. Prophages homologous to SWU1 were identified in two pathogens, Segniliparus rugosus ATCC BAA-974 and Mycobacterium rhodesiae JS60. Genus Segniliparus is a member of the order Corynebacteriales. To our knowledge, this is the first report of Mycobacterium prophages in different genera.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Virales
/
ADN Viral
/
Genoma Viral
/
Mycobacterium smegmatis
/
Micobacteriófagos
País/Región como asunto:
Asia
Idioma:
En
Revista:
Gene
Año:
2015
Tipo del documento:
Article