Spatial distribution of Auger electrons emitted from internalised radionuclides in cancer cells: the photoresist autoradiography (PAR) method.
Radiat Prot Dosimetry
; 166(1-4): 228-32, 2015 Sep.
Article
en En
| MEDLINE
| ID: mdl-25889606
ABSTRACT
Microdosimetric evaluation of Auger electron-emitting radionuclides involves a detailed evaluation of energy deposition at a nanometre scale. To perform Monte Carlo modelling of such energy deposition, accurate information regarding the spatial distribution of the radionuclide is required. A recent addition to the methods for determining the spatial distribution of cellular internalised radionuclides is based on detection in a polymer photoresist (e.g. polymethyl methacralate), followed by atomic force microscopy analysis of the resultant 3D pattern. In comparison with present practice, the method offers greater spatial resolution and improved quantification. The volume of the pattern is proportional to the total dose, thereby permitting assessment of variability of accumulated activity, while the variation in depth across the pattern reflects the lateral spatial distribution in the local fluence per unit area. An added advantage is the similarity in response to ionising radiation of an organic polymer compared to that of biological material. A pattern in the resist from radiation emitted by a radionuclide treated cell gives additional spatial information about the energy deposited in the resist.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Autorradiografía
/
Radioisótopos de Indio
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Carcinoma de Células Escamosas
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Microscopía de Fuerza Atómica
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Electrones
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Neoplasias de Cabeza y Cuello
Límite:
Humans
Idioma:
En
Revista:
Radiat Prot Dosimetry
Año:
2015
Tipo del documento:
Article
País de afiliación:
Reino Unido