A multivariate approach for high throughput pectin profiling by combining glycan microarrays with monoclonal antibodies.
Carbohydr Res
; 409: 41-7, 2015 May 29.
Article
en En
| MEDLINE
| ID: mdl-25950120
ABSTRACT
Pectin-one of the most complex biomacromolecules in nature has been extensively studied using various techniques. This has been done so in an attempt to understand the chemical composition and conformation of pectin, whilst discovering and optimising new industrial applications of the polymer. For the last decade the emergence of glycan microarray technology has led to a growing capacity of acquiring simultaneous measurements related to various carbohydrate characteristics while generating large collections of data. Here we used a multivariate analysis approach in order to analyse a set of 359 pectin samples probed with 14 different monoclonal antibodies (mAbs). Principal component analysis (PCA) and partial least squares (PLS) regression were utilised to obtain the most optimal qualitative and quantitative information from the spotted microarrays. The potential use of microarray technology combined with chemometrics for the accurate determination of degree of methyl-esterification (DM) and degree of blockiness (DB) was assessed.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacáridos
/
Pectinas
/
Análisis por Micromatrices
/
Anticuerpos Monoclonales
Tipo de estudio:
Qualitative_research
Idioma:
En
Revista:
Carbohydr Res
Año:
2015
Tipo del documento:
Article
País de afiliación:
Dinamarca