A simple method for expression and purification of Shiga toxin 1 (Stx1) with biological activities by using a single-promoter vector and native signal peptide.

Tu, Wei; Li, Tao; Wang, Qin; Cai, Kun; Gao, Xiang; Wang, Hui

Tu, Wei; Li, Tao; Wang, Qin; Cai, Kun; Gao, Xiang; Wang, Hui.

Afiliación

Tu W; State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Fengtai District, Beijing, People's Republic of China.
Li T; State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Fengtai District, Beijing, People's Republic of China.
Wang Q; State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Fengtai District, Beijing, People's Republic of China.
Cai K; State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Fengtai District, Beijing, People's Republic of China.
Gao X; State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Fengtai District, Beijing, People's Republic of China.
Wang H; State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Fengtai District, Beijing, People's Republic of China.

Biotechnol Appl Biochem ; 63(4): 539-45, 2016 Jul.

Article en En | MEDLINE | ID: mdl-26031547

Asunto(s)

Ingeniería Genética/métodos; Vectores Genéticos/genética; Regiones Promotoras Genéticas/genética; Señales de Clasificación de Proteína; Toxina Shiga I/química; Toxina Shiga I/genética; Animales; Chlorocebus aethiops; Femenino; Expresión Génica; Ratones; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/aislamiento & purificación; Proteínas Recombinantes/toxicidad; Análisis de Secuencia; Toxina Shiga I/aislamiento & purificación; Toxina Shiga I/toxicidad; Células Vero

Palabras clave

native signal peptide; recombinant Shiga toxin 1(rStx1); single promoter

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Señales de Clasificación de Proteína / Ingeniería Genética / Regiones Promotoras Genéticas / Toxina Shiga I / Vectores Genéticos Límite: Animals Idioma: En Revista: Biotechnol Appl Biochem Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2016 Tipo del documento: Article

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