NEUROSCIENCE. Natural light-gated anion channels: A family of microbial rhodopsins for advanced optogenetics.
Science
; 349(6248): 647-50, 2015 Aug 07.
Article
en En
| MEDLINE
| ID: mdl-26113638
ABSTRACT
Light-gated rhodopsin cation channels from chlorophyte algae have transformed neuroscience research through their use as membrane-depolarizing optogenetic tools for targeted photoactivation of neuron firing. Photosuppression of neuronal action potentials has been limited by the lack of equally efficient tools for membrane hyperpolarization. We describe anion channel rhodopsins (ACRs), a family of light-gated anion channels from cryptophyte algae that provide highly sensitive and efficient membrane hyperpolarization and neuronal silencing through light-gated chloride conduction. ACRs strictly conducted anions, completely excluding protons and larger cations, and hyperpolarized the membrane of cultured animal cells with much faster kinetics at less than one-thousandth of the light intensity required by the most efficient currently available optogenetic proteins. Natural ACRs provide optogenetic inhibition tools with unprecedented light sensitivity and temporal precision.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Canales de Cloruro
/
Rodopsinas Microbianas
/
Criptófitas
/
Optogenética
/
Potenciales de la Membrana
/
Neuronas
Límite:
Humans
Idioma:
En
Revista:
Science
Año:
2015
Tipo del documento:
Article
País de afiliación:
Estados Unidos