Generation of Cynomolgus Monkey Chimeric Fetuses using Embryonic Stem Cells.
Cell Stem Cell
; 17(1): 116-24, 2015 Jul 02.
Article
en En
| MEDLINE
| ID: mdl-26119236
ABSTRACT
Because of their similarity to humans, non-human primates are important models for studying human disease and developing therapeutic strategies. Establishment of chimeric animals using embryonic stem cells (ESCs) could help with these investigations, but has not so far been achieved. Here, we show that cynomolgus monkey ESCs (cESCs) grown in adjusted culture conditions are able to incorporate into host embryos and develop into chimeras with contribution in all three germ layers and in germ cell progenitors. Under the optimized culture conditions, which are based on an approach developed previously for naive human ESCs, the cESCs displayed altered growth properties, gene expression profiles, and self-renewal signaling pathways, suggestive of an altered naive-like cell state. Thus our findings show that it is feasible to generate chimeric monkeys using ESCs and open up new avenues for the use of non-human primate models to study both pluripotency and human disease.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Quimera por Trasplante
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Células Madre Embrionarias
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Macaca fascicularis
Tipo de estudio:
Prognostic_studies
Límite:
Animals
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Female
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Humans
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Male
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Pregnancy
Idioma:
En
Revista:
Cell Stem Cell
Año:
2015
Tipo del documento:
Article